MicroPoint provides a flexible and field-proven tool for photo-stimulation. Supplied with a patented compact, pulsed nitrogen pumped tunable dye laser it is capable of ablation, bleaching and uncaging over a wavelength range of 365 to 656 nm. Broad wavelength range and energy control allow MicroPoint to be optimized for a wide range of scenarios. More than 20 wavelengths can be utilized with available dye resonator cells, while appropriate dichroic filter sets enable simultaneous imaging and photo-stimulation of the specimen. MicroPoint is supplied with a UV-Vis imaging quality Epi illumination adapter for both current and previous generation microscopes from Leica, Nikon, Olympus and Zeiss.
MicroPoint systems consist of a wavelength tuneable pulsed laser, coupling optics, beam steering optics, a microscope adapter, a selection of beam splitters and interference filters, and a motorized or manually driven optical attenuator to adjust spot size and power. There are two MicroPoint versions available:
Angular and spatial alignment of the illumination at the sample target is manually controlled via a 2-axis joystick. This manual version can be upgraded in-situ to provide galvo or bluetooth control.
Galvanometer based beam steering is provided through PC control, enables precise and repeatable laser ablation and/or illumination in synchronization with other experiment parameters.
Simultaneous laser delivery, microscope viewing and image acquisition -
Low maintenance with fiber optic delivery that maintains alignment when system is moved -
Quick set-up with manual beam positioning or automatic pattern generation -
User control of ablation and illumination plane provided by z-axis telescope -
Precise control of energy provided by motorized variable attenuator slide -